Continuous purification of a parvovirus using two aqueous two-phase extraction steps.

ABSTRACT: Aqueous two-phase systems (ATPS) are a liquid-liquid extraction method that offers low-cost, continuous-adaptable virus purification. A two-step ATPS using polyethylene glycol (PEG) and sodium citrate that recovered 66% of infectious porcine parvovirus with 2.0 logs of protein removal and 1.0 logs of DNA removal in batch has now been run continuously. The continuous system output of <10 ng/mL DNA regardless of starting DNA titer agreed with batch studies. However, the continuous system had a five-fold higher contaminating protein titer than batch studies, likely because of incomplete mixing or settling. Turbidity was tested as a measure of mixing and settling efficiency. Monitoring in-line absorbance at 880 nm directly after mixing and before collection in the settling reservoir could trac

SUBMITTER: Nold NM 

PROVIDER: S-EPMC12531933 | biostudies-literature | 2025 Sep-Oct

REPOSITORIES: biostudies-literature