A cross-species multi-omics analyze uncovers conserved molecular mechanisms underlying age-related erectile dysfunction.

ABSTRACT:

Background

The urgent need for new treatments is driven by the challenging clinical situation of age-related erectile dysfunction (ARED).

Aim

To clarify the conserved molecular mechanisms of ARED across species using multi-omics.

Methods

Rat and mouse models with ARED were developed to facilitate the extraction of mRNA and proteins from the corpus cavernosum for high-throughput sequencing. Bioinformatics techniques were employed to analyze differentially expressed genes and to conduct analyses using the Kyoto Encyclopedia of Genes and Genomes, Gene Ontology, and protein-protein interaction networks. Verification of the results was carried out using immunofluorescence, hematoxylin-eosin staining, and Masson staining.

Outcomes

The multi-omics profiles of ARED rat

SUBMITTER: Long Q 

PROVIDER: S-EPMC12531994 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature