Multiprobe Photoproximity Labeling of the EGFR Interactome in Glioblastoma Using Red-Light.

ABSTRACT: Photocatalytic proximity labeling has emerged as a valuable technique for studying interactions between biomolecules in a cellular context, providing precise spatiotemporal control over protein labeling. One significant advantage of these methods is their modularity, allowing the use of a single photocatalyst with different reactive probes to expand interactome coverage and capture diverse protein interactions. Despite these advances, fewer methods have been developed using red-light excitation, limiting the use of photoproximity labeling in more complex media such as tissues and animal models. Herein, we develop a platform for proximity labeling under red-light excitation, utilizing a single catalyst and two distinct probe types. We first design a carbene based labeling system that utiliz

SUBMITTER: Tong F 

PROVIDER: S-EPMC12532021 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature