One-Step Label-Free Electrochemical Lateral Flow Immunosensor for SARS-CoV‑2 Antigen Detection.

ABSTRACT: Rapid and ultrasensitive diagnostic tests for COVID-19 remain crucial, yet conventional lateral flow antigen kits are limited by their reliance on labeled probes and suboptimal sensitivity at low viral loads. Here, we present a label-free electrochemical antigen test kit (free-EATK) that enables one-step detection of the SARS-CoV-2 N protein without the need for conjugate pads, covalently labeled redox probes, or signal normalization schemes. The system integrates a nitrocellulose-coated electrode, a redox pad preloaded with [Ru-(NH₃)₆]³⁺, and a sample inlet. Upon sample application, the immunocomplex forms directly at the sensing zone, followed by diffusion of the redox mediator toward the electrode surface. Signal generation is achieved through direct ano

SUBMITTER: Kumsab J 

PROVIDER: S-EPMC12532048 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature