PHEAA/pMA-DNA Hydrogel Sensing Platform for Detecting Drug-Resistant Gene Variants in <i>Mycobacterium tuberculosis</i>.

ABSTRACT: Mutations in the drug-resistant gene ofMycobacterium tuberculosiscan make it challenging to use drugs in clinical practice. Traditional genetic testing for resistance requires cell culture and susceptibility testing, which take 1-2 weeks. In this study, a DNA-sensitive hydrogel (pHEAA/pMA-DNA) has been developed with nucleic acid binding ability, water retention capacity, and high-temperature resistance, allowing it to work normally at 105 °C. Molecular dynamics simulations have been used to obtain the necessary physicochemical parameters. The DNA-sensitive hydrogel acts as a novel biosensor for detecting rifampicin- and isoniazid-resistant gene mutations in Mycobacterium tuberculosis. The microarray sensor's detection range is between 10⁹ copies/ml and 10¹

SUBMITTER: Sun T 

PROVIDER: S-EPMC12532050 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature