A Redefined Protocol for Protein Corona Analysis on Graphene Oxide.

ABSTRACT: It is well established that the biomolecular corona affects the biological behavior of nanomaterials, including cellular uptake, toxicity, and biodistribution. However, the unique physicochemical properties of advanced materials, such as graphene oxide, challenge the effectiveness of standard protocols for biomolecular corona characterization, which may lead to incomplete biomolecule recovery and biased experimental results. Protein analysis is one of the broadest techniques in the characterization of the biomolecular corona, providing important information about the composition and behavior of proteins adsorbed onto nanomaterial surfaces. Two widely accepted protein analysis methods include SDS-PAGE and mass spectrometry, and both require the complete elution of the proteins from the nano

SUBMITTER: Saorin A 

PROVIDER: S-EPMC12532052 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature