Precise Characterization of Individual Microfluidic Droplets Using Laser Diffraction.

ABSTRACT: The expanding use of microfluidic droplets and particles across disciplines, from biology to materials science, highlights the need for developing precise characterization methods. Conventional particle characterization based on light scattering typically relies on averaged data from multiple particles, which can lead to inaccuracies due to contamination from larger particles. To overcome this issue, we here present a versatile laser diffraction (LD) system for characterizing individual droplets and particles flowing in a poly-(dimethylsiloxane) (PDMS) microfluidic device. Our system, mounted on a commercial inverted microscope, facilitates the simultaneous estimation of both the diameter and the refractive index of microparticles and droplets of size 20-50 μm. The LD system captures the a

SUBMITTER: Masui S 

PROVIDER: S-EPMC12532057 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature