A One-Pot RT-LAMP Diagnostic Assay for SARS-CoV‑2 from Saliva Samples.

ABSTRACT: Results of efforts to diagnose infections with SARS-CoV-2 using a sampling method that was less invasive than the nasopharyngeal swab led to the rapid adoption of anterior nasal swabs. Saliva was also shown to have potential as a sample matrix and, like anterior nasal swabs, could be obtained noninvasively (e.g., passive drool). However, due to its inherent complexity and heterogeneity across patient populations (e.g., presence of mucins and RNases), saliva was largely disregarded as point-of-care diagnostics were being developed and broadly implemented. For molecular diagnostic approaches (e.g., RT-PCR or RT-LAMP), these matrix effects from saliva could lead to undesirable false positives or false negatives. The opportunity to address these challenges by normalizing the performance of sal

SUBMITTER: Mora AC 

PROVIDER: S-EPMC12532060 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature