Integrating High-Resolution Cyclic Ion Mobility Separations with Tandem Mass Spectrometry and Collision Cross Section Measurements for Human Milk Oligosaccharide Sequencing.

ABSTRACT: Human milk oligosaccharides (HMOs) are a biologically important class of carbohydrates responsible for promoting the healthy development of infants. However, to better understand their specific biological roles, analytical techniques are needed to unambiguously characterize them. While liquid chromatography-tandem mass spectrometry (LC-MS/MS) remains the gold standard for HMO analysis, new orthogonal techniques are desired for improving their isomer analysis. Ion mobility spectrometry-mass spectrometry (IMS-MS) has emerged as a complementary technique to LC-MS/MS but has seen little use toward HMO sequencing analysis beyond the construction of collision cross section (CCS) databases. In this work, we describe the use of collision-induced dissociation performed prior to high-resolution cycl

SUBMITTER: Habibi SC 

PROVIDER: S-EPMC12532065 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature