Probing human heart TCA cycle metabolism and response to glucose load using hyperpolarized [2-¹³ C]pyruvate MRS.

ABSTRACT:

Introduction

The healthy heart has remarkable metabolic flexibility that permits rapid switching between mitochondrial glucose oxidation and fatty acid oxidation to generate ATP. Loss of metabolic flexibility has been implicated in the genesis of contractile dysfunction seen in cardiomyopathy. Metabolic flexibility has been imaged in experimental models, using hyperpolarized (HP) [2-¹³ C]pyruvate MRI, which enables interrogation of metabolites that reflect tricarboxylic acid (TCA) cycle flux in cardiac myocytes. This study aimed to develop methods, demonstrate feasibility for [2-¹³ C]pyruvate MRI in the human heart for the first time, and assess cardiac metabolic flexibility.

Methods

Good manufacturing practice [2-¹³ C]pyruvic acid was polari

SUBMITTER: Chen HY 

PROVIDER: S-EPMC12532068 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature