First Evidence of Metabolically Active Intracellular Bacteria in <i>Saccharomyces cerevisiae</i>.

ABSTRACT: Quorum sensing (QS) is a cell-to-cell signaling system that takes place at a key concentration (quorum) of signal molecules and via a peculiar signaling pathway. Both bacteria and yeasts possess QS mechanisms, mediated by specific molecules (farnesol, tyrosol, 2-phenylethanol, tryptophol) in yeasts, and N-acylhomoserine lactones (AHLs) and modified oligopeptides in bacteria. Here, we report the first chemical evidence of bacterial QS activity in yeast Saccharomyces cerevisiae (OS3 and V5 strains) by UPLC-MS/MS identification of N-octanoyl- and N-decanoyl-L-homoserine lactones in cell-free culture media extracts. The AHLs' presence was unexpected, as they are produced exclusively by bacteria. Tyrosol, a yeast signal molecule, was identified and quantified by NMR

SUBMITTER: Tramice A 

PROVIDER: S-EPMC12532280 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature