Adaptive fluorescence lifetime imaging with per-pixel signal optimization and flexible scanning.

ABSTRACT: Advances in time-resolved fluorescence lifetime imaging microscopy (FLIM) have significantly enhanced biological imaging compared to steady-state techniques alone. The primary goal of modern FLIM is to acquire high-resolution fluorescence lifetime profiles with a high signal-to-noise ratio (SNR) from heterogeneous samples at high speeds, posing challenges in balancing imaging speed, signal strength, and sample integrity. In this study, we present fluorescence lifetime intensity-inverted imaging microscopy (FLI³M), an adaptive imaging technique based on confocal laser scanning microscopy (CLSM) that dynamically adjusts pixel dwell times using a priori intensity information from a pre-scan and supports flexible scanning patterns. This approach achieves uniform SNR imaging b

SUBMITTER: Xie S 

PROVIDER: S-EPMC12532327 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature