Megahertz dynamic optical coherence tomography of blisters in human skin.

ABSTRACT: Detecting epidermal blisters in human skin using optical coherence tomography (OCT) is clinically valuable, particularly for diagnosing autoimmune blistering diseases. Dynamic OCT (dOCT) extends conventional structural imaging by providing motion-based contrast sensitive to tissue dynamics. In this study, we analyze the diagnostic potential of dynamic contrast in high-speed (MHz-OCT) and microscopic (mOCT) OCT for blister imaging. We first evaluate whether these systems offer sufficient structural detail for blister detection, comparing them to a clinical reference. Dynamic contrast was then examined in an ex vivo human skin blister model using mOCT, and in vivo, MHz-OCT was subsequently applied to healthy and blistered skin. Our findings demonstrate improved layer delineatio

SUBMITTER: Gob M 

PROVIDER: S-EPMC12532343 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature