Residue 301-dependent epitope mapping reveals the molecular basis for GPV/MDPV serotype discrimination by neutralizing monoclonal antibody D1.

ABSTRACT: Waterfowl parvoviruses (goose parvovirus, GPV; Muscovy duck parvovirus, MDPV; Muscovy duck-origin goose parvovirus, MDGPV; short beak and dwarfism syndrome virus, SBDSV) are significant pathogens in waterfowl. The monoclonal antibody D1 specifically recognizes GPV-serotype parvoviruses (including GPV, SBDSV, and MDGPV) but cannot bind MDPV. Thus, Western blot and indirect immunofluorescence assays confirmed that D1 recognizes a conformational epitope, and residue 301 is critical for its discriminatory binding. Subsequent integrative approaches-combining AlphaFold 3-predicted structural modeling, molecular dynamics simulations, immunofluorescence, and transmission electron microscopy-revealed that VP3 residues 65 and 296 also contribute to the D1 epitope. Position 301 is a conserved diverge

SUBMITTER: Xiao S 

PROVIDER: S-EPMC12532459 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature