An optimized DNA extraction protocol for reliable PCR-based detection and characterization of grapevine flavescence doree phytoplasma.

ABSTRACT:

Background

Flavescence dorée (FD) is one of the most damaging grapevine diseases in Europe, caused by the quarantine-listed Grapevine flavescence dorée phytoplasma (FDp). Given the absence of resistant cultivars and curative treatments, effective disease control relies on early and accurate FDp detection. PCR-based diagnostics are the gold standard, but their accuracy depends on DNA extraction quality. Grapevine tissues contain PCR inhibitors like polysaccharides and polyphenols, complicating DNA isolation. While CTAB methods yield high-quality DNA, they are time-consuming, and commercial kits provide purer but often lower DNA yields at high costs. A rapid and optimized DNA extraction method for FDp detection is urgently needed.

Results

We developed the "HotShot Vitis" (HSV)

SUBMITTER: Carli M 

PROVIDER: S-EPMC12532460 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature