Characterisation and optimisation of microbial production of transglutaminase produced by Streptoverticillium cinnamoneum.

ABSTRACT: Optimal conditions for the production of microbial transglutaminase (MTG) by Streptoverticillium cinnamoneum KKP 1648 under bioreactor conditions were determined using response surface methodology (RSM). The test medium contained soluble starch, Aminobak, waste corn steep liquor, yeast extract, and mineral salts such as KH₂PO₄, Na₂HPO₄∙12H₂O, and MgSO₂∙7H₂O. The culture was conducted at 28 °C, stirring at 200 rpm, using aeration with compressed air at a flow rate of 2.0 vvm. Optimal culture parameters included a pH of 6.5, a nitrogen dose of 2.75%, and an incubation time of 72 h, and yielded MTG activities of 4.29 ± 0.08 and 22 ± 0.05 U/mL after precipitation with ammonium sulfate. The molecular weight of the enzy

SUBMITTER: Kolotylo V 

PROVIDER: S-EPMC12532617 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature