QuEChERS analytical approach for monitoring endocrine-disrupting chemicals in human urine.

ABSTRACT: This study introduces an analytical method for the determination of endocrine-disrupting chemicals (EDCs) in human urine samples, employing QuEChERS as a clean-up step for the first time in this biological matrix. Sample preparation was rigorously optimized through the evaluation of sample and final dilution volumes, balancing the need to reduce matrix interference without sacrificing sensitivity. High-performance liquid chromatography coupled with a triple quadrupole-time-of-flight mass spectrometer (HPLC-QTOF) was employed, enabling the detection of 13 target analytes (five OPEs, five phthalates, and three parabens' metabolites). Chromatographic separation was achieved in 16 min, and mass spectrometry parameters were fine-tuned to maximize signal sensitivity. The method was validated by

SUBMITTER: Callejas-Martos S 

PROVIDER: S-EPMC12532665 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature