Significance of chondrocyte viability in postmortem interval assessments and chondrocyte viability assay.

ABSTRACT: Determination of the postmortem interval (PMI) is one of the most challenging problems in forensic medicine. The main aim of this study was to determine the dynamics of the decrease in the fraction of viable chondrocytes excluded from the donors' knees for PMI determination. The other aim was to find an appropriate method for chondrocyte viability assay. We analyzed osteochondral cylinders from 35 donors (28 males and 7 females), aged 44 to 90 years, whose bodies were stored in refrigerators at temperature of 8 ± 2 °C, during the period from 4 to 83 days postmortem. The proportion of viable chondrocytes was determined by flow cytometry (FC) and cell viability analyzer (CVA). For FC we used RedDot™1 to mark all chondrocytes with nuclei (method FCN) and 7-AAD, to distinguish live/dead cells

SUBMITTER: Mihic AG 

PROVIDER: S-EPMC12532684 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature