Design of Novel Saposin-like Bacteriocins Using a Hybrid Approach.

ABSTRACT: A multitude of approaches will be required to respond to the threat posed by the emergence and spread of antibiotic resistant pathogens. Bacteriocins have gained increasing attention as a possible alternative to antibiotics, as such peptide antimicrobials have mechanisms of action different from antibiotics and are therefore equally potent against antibiotic resistant bacteria as their susceptible counterparts. A group of bacteriocins known as saposin-like bacteriocins is believed to act directly on the bacterial membrane. Based on seven saposin-like leaderless bacteriocins, we have constructed a library of hybrid peptides containing all combinations of the N- and C-terminal halves of the native bacteriocins. All hybrid peptides were synthesized using in vitro protein expression and assaye

SUBMITTER: Oftedal TF 

PROVIDER: S-EPMC12532754 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature