PARP1 contributes to intimal hyperplasia by regulating METTL3-mediated m6A methylation of TRAIL.

ABSTRACT: Poly (ADP-ribose) polymerase 1 (PARP1) promotes vascular intimal hyperplasia (IH) while contributing to N6-methyladenosine (m6A) methylation regulatory processes. The present study focuses on whether the PARP1 inhibitor PJ34 can improve vascular IH by regulating m6A methylation modification. Mice with femoral artery wire injury-induced IH and platelet-derived growth factor-BB (PDGF-BB)-challenged mouse vascular smooth muscle cells (VSMCs) were utilized in the study. PJ34 treatment significantly alleviated neointimal formation, suppressed VSMC proliferation and phenotypic switching, and reduced global m6A methylation and methyltransferase-like 3 (METTL3) expression in injured arteries. Dot blot, RT-qPCR, western blot, and immunohistochemistry confirmed these changes. In vitro, PJ34 impaired

SUBMITTER: Li S 

PROVIDER: S-EPMC12532763 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature