Fusobacterium nucleatum enhances oxaliplatin resistance in colon cancer by increasing PVT1 expression.

ABSTRACT:

Background

Approximately 50% of colorectal cancer (CRC) patients exhibit high levels of Fusobacterium nucleatum (Fn), which is associated with chemotherapy resistance. As Fn itself cannot be directly targeted for therapy in vivo, elucidating the mechanism underlying Fn-induced chemotherapy resistance is crucial, though it remains unclear.

Methods

qPCR was used to analyze the correlation between Fn abundance and clinical parameters in 80 human colon cancer samples. The effect of Fn on the sensitivity of colon cancer cells to oxaliplatin was evaluated by clone formation, EdU proliferation and apoptosis assays. RNA sequencing was performed on Fn-infected colon cancer cells to identify differentially expressed genes. Mechanistic studies explored the interaction between PVT1 and

SUBMITTER: Gao K 

PROVIDER: S-EPMC12532874 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature