Identifying the quantity profiles of amyloid signature proteins in different types of renal amyloidosis.

ABSTRACT:

Background

The aim of this study is to explore the quantity profiles of amyloid signature proteins (serum amyloid P component, SAP; apolipoprotein E, ApoE; apolipoprotein A-IV) in common types of renal amyloidosis by mass spectrometry and immunostaining methods.

Methods

Twenty-one patients with renal amyloidosis of different types evaluated at the Renal Pathological Center of Peking University First Hospital from 2000 to 2021 were enrolled. Immunohistochemistry (IHC) and laser microdissection combining with mass spectrometry (LMD-MS) were applied to investigate the localization and quantity profiles of signature proteins in renal amyloidosis. The co-localization relationships among signature proteins and amyloid fibrils, as well as the ultrastructural localization of SAP wer

SUBMITTER: Wang S 

PROVIDER: S-EPMC12532953 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature