Exploring the pathogenesis of steroid-induced osteonecrosis of the femoral head co-regulated by mesenchymal stem cells and pyroptosis-related genes with the use of transcriptome.

ABSTRACT:

Purpose

Mesenchymal stem cells (MSCs) and pyroptosis play an important part in steroid-induced osteonecrosis of the femoral head (SONFH). The present study aimed at the identification and analysis of MSC and pyroptosis-related biomarkers for the treatment of SONFH, as well as the elucidation of their biological mechanisms.

Methods

The present study first use the outliers in GSE123568 data set were removed by principal component analysis (PCA). Next, differentially expressed genes (DEGs) in GSE123568 were identified using different expression analysis. Then, immune infiltration analysis and Wilcoxon test were performed. Later, acquires the DE_MSCs between DEGs and MSC, and DE_PRGs between DEGs and PRGs score. After, the top 20 intersected genes were selected by overlapping th

SUBMITTER: Rong XB 

PROVIDER: S-EPMC12532964 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature