Uncovering the anti-cervical cancer mechanism of Ziyuglycoside I via integrated network pharmacology molecular docking and experimental validation.

ABSTRACT: We aimed to conduct a systematic analysis of the multi-target regulatory mechanisms of Ziyu glycoside I (Ziyu I) in cervical cancer progression using network pharmacology and molecular docking techniques. Additionally, we validated our findings using cellular experiments and animal models. The PhamMapper database was used to identify drug targets for Ziyu I, while cervical cancer-related disease targets were obtained from the DisGeNET, DrugBank, GeneCards, and OMIM databases. In vitro, the effects of Ziyu I on the proliferation, migration, apoptosis, and cell cycle were assessed using cultured HeLa and SiHa cervical cancer cells. Network pharmacology analysis identified 68 potential targets of Ziyu I in cervical cancer. Molecular docking findings revealed that Ziyu I had a stable binding a

SUBMITTER: Huang S 

PROVIDER: S-EPMC12532998 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature