PyPlaque is an open-source python package for phenotypic analysis of virus plaque assays.

ABSTRACT: Virological plaque assays are the primary method for quantifying infectious particles in a suspension, achieved by incubating a serial dilution of the virus with a monolayer of indicator cells. Existing software tools for quantification of plaque assay images lack modularity, show measurements disagreement or are closed-source - a common hurdle in bioimage analysis. We introduce PyPlaque, an open-source Python package focusing on flexibility and modularity rather than a bulky graphic user interface. Unlike previous methods, an abstracted architecture using object-oriented programming allows accommodation of various experimental containers and specimen carriers as data structures while focusing on phenotype-specific information. Aligned with the logical flow of experimental design and desir

SUBMITTER: De T 

PROVIDER: S-EPMC12533079 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature