Long-term atorvastatin improves cognitive function by modulating SIRT2-mediated dynamic transition of NFL lysine 272 crotonylation to ubiquitination in naturally aging rats.

ABSTRACT: Proteins' reversible post-translational modifications (PTMs) are essential for cellular regulation, but their involvement in statin-mediated neuroprotection remains elusive. Our previous research demonstrated that long-term atorvastatin intervention ameliorates cognitive decline in naturally aged rats, although the underlying mechanisms were unknown. Here, we employed a proteomic and PTMomic approach using mass spectrometry-based quantitative proteomics and bioinformatics analyses to elucidate the molecular underpinnings. We also utilized isolated and cultured rat hippocampal neuronal cells to investigate the regulation of neurofilament light chain (NFL) modifications, including crotonylation and ubiquitination, using techniques such as immunoprecipitation, cell transfection, protein impri

SUBMITTER: Xu TC 

PROVIDER: S-EPMC12533114 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature