H<sub>2</sub>O<sub>2</sub>-Activated Serotonin Precursor Probe for Mapping Neuronal Redox Homeostasis Reveals 5-HT Interactions with Neighboring Proteins Under Oxidative Stress.
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ABSTRACT: Serotonin (5-HT) is a critical neurotransmitter that regulates various neurophysiological processes. However, the role of 5-HT under oxidative stress remains largely unexplored. Here, the development of a novel intramolecular charge transfer (ICT)-based fluorescent probe is reported, termed HOP, designed using a tandem sensing and labeling strategy. HOP is selectively activated by hydrogen peroxide (H2O2) in neuronal cells undergoing oxidative stress. Upon activation, HOP emits fluorescent signals and covalently cross-links with nearby proteins, which not only anchors it to the local microenvironment to avoid diffusion of the fluorophore, but also simultaneously releases 5-HT in situ. The locally released 5-HT further interacts with nearby functional proteins such as
SUBMITTER: Liu Y
PROVIDER: S-EPMC12533203 | biostudies-literature | 2025 Oct
REPOSITORIES: biostudies-literature
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