O-GlcNAc transferase influences the progression of degenerative cartilage disease in lumbar facet joint osteoarthritis through FoxO1 and EGR1.

ABSTRACT: This study aimed to investigate the role of the O-GlcNAc transferase (OGT)/Forkhead Box O1 (FoxO1) signaling axis in age-induced facet joint osteoarthritis (FJOA) and its impact on chondrocyte homeostasis. Using a mouse model, the effects of OGT knockout on cartilage integrity in facet joints were examined. Molecular assays were conducted to analyze OGT's influence on FoxO1 expression and its stability against ubiquitin-mediated degradation. In vitro studies with chondrocytes were performed to assess the impact of FoxO1 overexpression on extracellular matrix synthesis, while in vivo experiments were carried out to test the protective effects of FoxO1 overexpression on FJOA progression. Additionally, the functionality of ATDC5 cells and the regulation of EGR1 by the OGT/FoxO1 axis were eval

SUBMITTER: Chen C 

PROVIDER: S-EPMC12533218 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature