Identification and validation of SQLE in steroid-induced osteonecrosis of the femoral head: a bioinformatics and experimental study.

ABSTRACT:

Background

Osteonecrosis of the femoral head (ONFH) is a prevalent and refractory hip disease. In this study, we investigated the expression profiles of lipid metabolism-related genes in ONFH and evaluated the potential therapeutic effects of squalene epoxidase (SQLE) and its inhibitors.

Methods

Dexamethasone was used to establish an in vitro ONFH model in MC3T3-E1 cells. Differentially expressed genes (DEGs) in the model group were identified through transcriptome sequencing. Lipid metabolism-related DEGs were extracted from GeneCards, and hub genes were determined via a protein-protein interaction (PPI) network. The expression patterns and diagnostic value of these hub genes were further validated using the GEO dataset. qRT-PCR and WB were performed to detect the expressio

SUBMITTER: He Y 

PROVIDER: S-EPMC12533337 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature