Near-Infrared-Responsive Digital PCR-Assisted Renal Cancer Exosomal miRNAs Insights and Regulation of Macrophage Polarization.

ABSTRACT: Exosomes derived from tumors are critical agents in intercellular communication and the tumor microenvironment, offering a rich source of signatures for renal cell carcinoma (RCC) diagnosis. Conventional diagnostic techniques often suffer from limited sensitivity and can be invasive. This study presents an innovative approach using near-infrared (NIR) digital PCR (dPCR) with black phosphorus-embedded gelatin microcarriers for profiling exosomal miRNAs and modulating STAT3 signaling and macrophage polarization. Microcarriers produced via microfluidics, characterized by their phase-change and photothermal properties, are subjected to thermal cycling using a custom NIR source. The study identified a 4.2-fold increase in miR-210 levels in RCC cells (ACHN and A498) compared to normal cells (HK-

SUBMITTER: Zhang L 

PROVIDER: S-EPMC12533400 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature