UPLC-ESI-QTOF-MS/MS Profiling, Antioxidant, and Cytotoxicity Potentials of Marrubium vulgare L. Extracts: Experimental Analysis and Computational Validation.

ABSTRACT: Plant extracts are emerging as valuable options for food additives and therapeutic treatments. This study evaluated the phytochemical profile, antioxidant activity, and cytotoxicity of aerial parts of Marrubium vulgare L. crude extract (MVCE) and its subfractions. The MVCE (80% ethanol) contained steroids, phenolic compounds, flavonoids, terpenes, and cardiac glycosides, with total phenolic content (TPC) and total flavonoid content (TFC) of 14.96 ± 0.12 mg GAE/g DW and 12.27 ± 0.63 mg RE/g DW, respectively. All MV extracts exhibited potent antioxidant activity against DPPH^• (0.106-1.864 mg/mL) and ABTS^+• (0.298-17.084 mg/mL). The MV residual aqueous fraction (MVRF) showed significant cytotoxicity against human cancer cell lines, including MCF7 (IC₅₀ = 5.47 

SUBMITTER: Mannoubi IE 

PROVIDER: S-EPMC12533807 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature