Impact of substrate choice on human osteoclast differentiation and secretome: Implications for targeted therapeutic development.

ABSTRACT: Osteoclasts (OCs) exhibit substrate-specific molecular adaptations crucial for bone remodeling. We utilized mass spectrometry and functional enrichment analysis to delineate the proteomic profiles of mature polarized OCs cultured on mineralized versus plastic plates. Our findings reveal that mineralized surfaces promote the expression of proteins specialized for bone resorption and matrix interaction, such as lysosomal enzymes and ion transporters. This environment induces a mature and resorptive phenotype in OCs, enriched in pathways like VEGF/VEGFR signaling and various cytokine pathways. Conversely, OCs on plastic plates display a more diverse proteomic profile, highlighting adaptations in adhesion, proliferation, and stress response pathways, suggesting a focus on cellular maintenance

SUBMITTER: D'Amelio F 

PROVIDER: S-EPMC12533897 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature