Expansion of the functional genomics GRACE library reveals genes relevant for temperature-dependent fitness in Candida albicans.

ABSTRACT: A small percentage of species in the fungal kingdom can cause devastating infections in humans, with Candida albicans reigning as a leading cause of systemic disease. One of the key virulence phenotypes for pathogenic fungi is the ability to survive at host body temperature; however, a comprehensive understanding of the mechanisms that orchestrate thermal adaptation in fungi remains incomplete. In this study, we expand the largest functional genomics resource in C. albicans, reaching 71.3% coverage of the entire genome, and perform screens under six different temperatures to identify genes important for temperature-dependent fitness. We describe the function of genes involved in translation (GAR1), splicing (C1_11680C or YSF3), and cell cycle progression (C6_00110C or RHT1) in enabling fun

SUBMITTER: Fu C 

PROVIDER: S-EPMC12533916 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature