Recombinant <i>Mycobacterium smegmatis</i> expressing antigen 85B for immunotherapy of <i>Mycobacterium tuberculosis</i>-infected mice.

ABSTRACT: Tuberculosis is a serious, chronic infectious disease worldwide, primarily caused by Mycobacterium tuberculosis (Mtb). Antigen 85B is a potential vaccine candidate for Mtb. In this study, we constructed a recombinant strain expressing Antigen 85B (rMs-Ag85B) in the Mycobacterium smegmatis (Ms). The immunological characteristics and the therapeutic efficacy of the vaccine were evaluated in mice. The results showed that rMs-Ag85B exhibited slow growth under static culture conditions, with a significant reduction in biofilm formation compared to the Ms strain. Immunologically, subcutaneous immunization with rMs-Ag85B induced stronger humoral and cellular immune responses. As a therapeutic vaccine, rMs-Ag85B modulated the aggregation of CD4⁺ and CD8⁺ T cells

SUBMITTER: Chang Y 

PROVIDER: S-EPMC12533959 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature