ScRNA+TCR-seq reveals the proportion and characteristics of dual TCR Treg cells in mouse lymphoid and non-lymphoid tissues.

ABSTRACT: The rearrangement of TCR germline V(D)J genes during T cell development, including allelic exclusion and tolerance selection, ensures the clonal selection theory, which states that 'a lymphocyte expresses only one type of antigen receptor'. This forms the basis for T cell-specific responses. However, the existence of 'dual TCR T cells' has consistently been supported by specific experimental evidence. Detailed reports on the origin, proportion, tissue distribution, and CDR3 characteristics of 'dual TCR Treg cells' are currently lacking. In this study, we utilized scRNA+TCR-seq technology to achieve in-depth analysis of single and dual TCR T pairings, along with their mRNA expressions, from over 5000 T cells in each sample. Through comparative studies with shared databases, we provided a de

SUBMITTER: Xu Y 

PROVIDER: S-EPMC12534043 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature