Nanopore detection of single-nucleotide RNA mutations and modifications with programmable nanolatches.

ABSTRACT: RNA mutations and modifications have been implicated in a wide range of pathophysiologies. However, current RNA detection methods are hindered by data complexity and error-prone protocols, restricting their widespread use. Here we present a solid-state nanopore-based approach, RNA single-nucleotide characterization and analysis nanolatch (RNA-SCAN) system, which simplifies the detection of nucleotide mutations and modifications in RNA with high resolution. Using phage RNA as a template, we tested multiple sequences and chemical modifications on nanolatches, allowing the detection of mismatches caused by nucleotide mutations through significant changes in positive event ratios using single-molecule nanopore measurements. This approach is also sensitive to modifications that either strengthe

SUBMITTER: Li Y 

PROVIDER: S-EPMC12534182 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature