Antioxidant Properties of Whey Protein-Derived Peptides: Radical Scavenging and Cytoprotective Effects.

ABSTRACT: Whey protein hydrolysates (WPH) are rich in peptides with potential antioxidant properties, yet the specific contributions of individual sequences remain underexplored. In this study, we identified eight antioxidant peptides-GYDTQ, GY, VY, DTDYKKY, WYS, WY, LDQW, and YW-from WPH by isolating fractions with high hydrophilic-oxygen radical absorbance capacity (H-ORAC) activity. Among them, GYDTQ was identified as a novel antioxidant peptide. The antioxidant activities of these peptides were further evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2, 2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays. All eight peptides exhibited radical scavenging activity: LDQW showed the highest H-ORAC value (3.79 ± 0.15 μmol Trolox equivalents [TE]/μmol), YW demo

SUBMITTER: Suzuki R 

PROVIDER: S-EPMC12534199 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature