Green synthesis of silver nanoparticles from Bacillus subtilis-mediated feather hydrolysate: antimicrobial, larvicidal against culex pipiens, and anticancer activities.

ABSTRACT: This study presents a novel dual-stage bioprocessing approach that transforms poultry feather waste into multifunctional silver nanoparticles (FWH-AgNPs) with enhanced bioactivity. Bacillus subtilis degradation of feather waste produced bioactive hydrolysate (FWH) with dramatically altered chemical composition, generating novel compounds including 9,12,15-octadecatrienoic acid methyl ester (25.66%) and cyclopropaneoctanoic acid methyl ester (23.02%). The FWH effectively synthesized spherical AgNPs (30-69 nm) with strong colloidal stability (-44.5 mV zeta potential) and characteristic surface plasmon resonance (420 nm). FWH-AgNPs demonstrated superior antimicrobial efficacy with 4-eightfold improved minimum inhibitory concentrations against Pseudomonas aeruginosa (125 μg/mL), methicillin-re

SUBMITTER: Alruhaili MH 

PROVIDER: S-EPMC12534213 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature