The pyrrolizidine alkaloid lasiocarpine impairs cell cycle progression in vitro.

ABSTRACT: 1,2-Unsaturated pyrrolizidine alkaloids (PA) induce severe acute and chronic hepatotoxicity. Effects include an impairment of the cell cycle. To elucidate this effect, cell cycle progression was analyzed by flow cytometry, changes in cell and nucleus size, differential gene expression and protein phosphorylation patterns of regulatory key proteins in the Chinese hamster lung fibroblast cell line V79 and/or the human hepatoblastoma cell line HepG2, both overexpressing human CYP3A4 (V79_3A4/HepG2_3A4). Lasiocarpine, a potent PA representative, reduced the cell viability of human CYP3A4-overexpressing cell lines concentration-dependently. Microscopic observation showed a marked increase in cell and nucleus size of V79_3A4 cells after exposure to 10 µM lasiocarpin

SUBMITTER: Hessel-Pras S 

PROVIDER: S-EPMC12534242 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature