Changes in ferroptosis-related molecular expression in macrophages of community-acquired pneumonia patients and their associated with the disease progression.

ABSTRACT:

Objective

To investigate the expression of ferroptosis-related molecules in macrophages of community-acquired pneumonia (CAP) patients and explore their association with disease severity. This study provides novel insights into the role of ferroptosis in CAP pathogenesis by integrating transcriptome sequencing, RT-qPCR, Western blot, and flow cytometry analyses.

Methods

Ferroptosis-related molecules were analyzed in CD14⁺ monocytes and CD11b⁺ macrophages from CAP patients using transcriptome sequencing, RT-qPCR, Western blot, and flow cytometry. Clinical data from CAP patients (n = 46) and healthy controls (n = 63) were compared.

Results

CAP patients exhibited elevated levels of ferroptosis markers (PTGS2, Fe²⁺, and lipid peroxides) and downregulation of inhibitors (T

SUBMITTER: Huang HS 

PROVIDER: S-EPMC12534296 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature