Mature CD209⁺CD83⁺CCR7⁺ dendritic cells infiltrate the arterial wall in giant cell arteritis and derive from in-situ monocyte differentiation.

ABSTRACT: This study aimed to characterize arterial dendritic cells (DCs) in polymyalgia rheumatica (PMR) and giant cell arteritis (GCA). Bulk RNA-sequencing, RT-PCR and immunofluorescence analyses were performed from temporal arteries from GCA, PMR and control patients. Public single-cell RNA-seq (scRNA-seq) data on Peripheral Blood Mononuclear Cells (PBMCs) were analyzed from three GCA and three control patients. Bulk RNA-Seq and RT-PCR analyses demonstrated a high level of expression of DC lineage markers (CD209), DC maturation markers (CD83, CCR7) and chemokines associated with DC maturation in GCA arteries. The level of expression of DC lineage and DC maturation associated genes was significantly lower in PMR than in GCA arteries and similar between PMR and control arteries. GCA arteries expres

SUBMITTER: Ramon A 

PROVIDER: S-EPMC12534448 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature