Validating a remote saliva collection tool for genomic analyses in free ranging dogs.

ABSTRACT: Saliva is a well-established source of DNA for various applications due to its non-invasive collection and its provision of high-quality DNA. However, its use in wild and free-ranging animal research remains limited due to challenges in collection without direct animal handling. In this study, we developed and evaluated a hands-off saliva collection method designed for free-ranging domestic dogs (FRDs), serving as a model for non-invasive genetic sampling of wildlife. Our method utilized a funnel paired with a commercially available Performagene kit (DNA Genotek, Canada), presented to the dog in the presence of an operator. The dog was free to approach and interact with the apparatus, depositing saliva while trying to reach bait. We compared DNA yield and genotyping success from samples us

SUBMITTER: Cimarelli G 

PROVIDER: S-EPMC12534496 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature