RHOBTB2 enhances cell proliferation of acute myeloid leukemia by modulating Hippo-YAP1 signaling and dependent of KLHL13.

ABSTRACT: Acute myeloid leukemia (AML) is a heterogeneous hematologic malignancy associated with poor clinical outcomes. RHOBTB2, an atypical member of the Rho-GTPase family, contains a conserved GTPase domain at its N-terminus. While previous studies have implicated RHOBTB2 in AML progression, its underlying mechanisms remain inadequately defined. In this study, bioinformatics analyses revealed that RHOBTB2 is markedly upregulated in AML and correlates with unfavorable prognosis. To elucidate its functional role, we overexpressed or silenced RHOBTB2 in human AML cell lines KG-1 and MOLM-13. Functional assays, including CCK-8, Transwell, and Annexin V/PI staining, demonstrated that RHOBTB2 overexpression enhanced proliferation and migration, suppressed apoptosis, and shortened G0/G1 phase. Conversel

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12534508 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature