Spotlighting the criticality of lipid quality control through a mechanistic investigation of mRNA activity loss in lipid nanoparticles.

ABSTRACT: Lipid nanoparticles (LNPs) have gained much attention after the recent launch of mRNA based Covid vaccines. For mRNA encapsulated within LNPs to be successfully translated into target proteins, the mRNA must maintain its integrity and also be free of any unintended chemical modifications. Any process or raw material related impurities-and their degradation products-pose risks of chemically modifying mRNA, thereby affecting the quality of the final product. Given its inherent chemical reactivity and close association with the LNP lipids, encapsulated mRNA is especially susceptible to modifications by reactive impurity species present in the lipids. In our recent efforts to understand mRNA-lipid interactions within LNPs, we observed that the degradants of labile lipid peroxide species in ion

SUBMITTER: Kang RS 

PROVIDER: S-EPMC12534529 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature