Immune cell dynamics and prognostic role of M2 macrophages and LDOC1 in colon adenocarcinoma.

ABSTRACT:

Background

Colon adenocarcinoma (COAD) is influenced by the tumor microenvironment, including immune cell dynamics, particularly M2 macrophages. Understanding these dynamics can offer insights into tumor progression and potential therapies.

Methods

We analyzed TCGA COAD data to quantify immune infiltration using TIMER, CIBERSORT, QUANTISEQ, MCPcounter, xCell, and EPIC methods. The ESTIMATE algorithm provided stromal and immune scores. WGCNA identified key gene modules correlated with immune infiltration. Prognostic significance of these genes was assessed using univariate Cox regression, Kaplan-Meier analysis, and nomogram construction. Single-cell RNA sequencing data from TISCH2 was used to explore gene expression correlations across cell types. We also examined correlation

SUBMITTER: Luo L 

PROVIDER: S-EPMC12534630 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature