Fusarium solani Activates PANoptosis and Modulates Immune Response in Fungal Keratitis.

ABSTRACT:

Purpose

This study aimed to investigate how Fusarium solani triggers PANoptosis in fungal keratitis and to examine the toxic impacts and underlying mechanisms of deoxynivalenol (DON) on human corneal epithelial (HCE-T) cells.

Methods

A F. solani keratitis mouse model was established through intrastromal injection of fungal spores. Clinical severity was evaluated using a standardized scoring system. PANoptosis markers in corneal tissues were analyzed via western blotting, immunofluorescence, and TUNEL assay. Real-time PCR was used to quantify inflammatory cytokines. RNA sequencing was conducted to select differentially expressed genes (DEGs) and analyze immune cell infiltration profiles. HCE-T cells were exposed to DON, and PANoptosis-related proteins were detected via wester

SUBMITTER: Sha X 

PROVIDER: S-EPMC12534895 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature