Tracking SARS-CoV-2 seroprevalence in frontline healthcare workers at the beginning of COVID-19 pandemic and reliability of serological laboratory interpretation: a prospective cohort study.

ABSTRACT:

Background

Frontline healthcare workers (HCWs) faced a high risk of SARS-CoV-2 infection during the early stages of the COVID-19 pandemic. This study aims to determine the prevalence and incidence of SARS-CoV-2 infection in HCWs using various serological methods and to evaluate diagnostic reliability.

Methods

A prospective study was conducted on 240 frontline and 120 non-frontline HCWs at Siriraj Hospital in Bangkok, Thailand, from May 2020 to March 2021. Blood samples were collected at enrollment and at 4, 12 and 48 weeks. Three serological methods were utilized: electrochemiluminescence immunoassay (ECLIA) for qualitative anti-nucleocapsid protein antibodies, enzyme-linked immunosorbent assay (ELISA) and chemiluminescent microparticle immunoassay (CMIA) for quantitative an

SUBMITTER: Sewatanon J 

PROVIDER: S-EPMC12535064 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature