LncRNA Rmst-driven DNMT3A mRNA stabilization through HuR: implications for neuropathic pain therapy.

ABSTRACT:

Objective

This study aimed to explore the role of lncRNA Rmst in regulating DNA methyltransferase 3A (DNMT3A) expression and its impact on neuropathic pain (NP).

Methods

A SAM-based lncRNA library screening system was employed to identify lncRNAs regulating DNMT3a stability. Spared nerve injury (SNL) and chronic constriction injury (CCI) rat models of neuropathic pain were established. LncRNA Rmst was knocked down in injured dorsal root ganglia via microinjection of siRmst. Lentiviral vectors carrying siRmst or pcDNA-DNMT3A were constructed and injected into mouse spinal cords. Analyses included qRT-PCR, Western blot, in situ hybridization, immunofluorescence, dual-luciferase reporter assays, and RNA immunoprecipitation (RIP). Pain-related behaviors were assessed using behav

SUBMITTER: Zhang S 

PROVIDER: S-EPMC12535087 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature