A Bacillus thuringiensis isolation method utilizing a novel stain, low selection and high throughput produced atypical results.

ABSTRACT:

Background

Bacillus thuringiensis is a bacterium known for producing protein crystals with insecticidal properties. These toxins are widely sought after for controlling agricultural pests due to both their specificity and their applicability in transgenic plants. There is great interest in isolating strains with improved or novel toxin characteristics, however isolating B. thuringiensis from the environment is time consuming and yields relatively few isolates of interest. New approaches to B. thuringiensis isolation have been, and continue to be sought. In this report, candidate B. thuringiensis isolates were recovered from environmental samples using a combination of a novel stain, high throughput and reduced selection. Isolates were further characterized by SDS-PAGE, light micros

SUBMITTER: Rampersad J 

PROVIDER: S-EPMC1253514 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature