Serum proteomic profiling during the periovulatory period identifies preliminary candidate biomarkers of oocyte maturation in deslorelin-induced ovulation in dogs.

ABSTRACT:

Background

The reproductive physiology of canines is unique from other mammals because oocyte maturation occurs about 48-72 hours after ovulation. This study aimed to evaluate the blood serum protein profile in canines during the periovulatory period by using shotgun proteomics to identify potential biomarkers of oocyte maturation.

Method

Anestrus female dogs (n = 9) were implanted subcutaneously with 4.7 mg of deslorelin to induce estrus and ovulation. After implantation, ovariectomy was performed based on the level of progesterone and vaginal cytology evaluations conducted every 48 hours. Simultaneously, serum samples were collected for proteomic analysis. The oocytes were flushed from the oviduct, and the oocyte maturation stage was identified. Based on oocyte stag

SUBMITTER: Udomthanaisit L 

PROVIDER: S-EPMC12535230 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature